Establishing Laboratory Analysis Procedure for EPA FIFRA Enforcement Samples to Meet the ISO/IEC 17025:2005 Requirements using Flexible Scope Approach

Yoko Shibuya Johnson, Gareth Horvath


An ISO/IEC 17025:2005 accredited laboratory procedure that quantifies analytes and validates an analytical method used simultaneously was established for analyzing pesticide misuse samples. Under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), the U.S. Environmental Protection Agency (EPA) grants the authority over regulating pesticide use to each state. Samples for pesticide misuse cases such as drift complaints are analyzed by state laboratories (state FIFRA labs). State FIFRA labs receive samples that require quick turnaround with a variety of unique matrix/analyte combinations. The majority of the samples are analyzed by non-standard methods that are developed by the laboratory. It is impractical to pre-validate such non-standard methods. Yet, the forensic nature of pesticide misuse investigation samples demands legally defensible analytical data. The author's laboratory developed an expedited validation procedure that is conducted simultaneously with analyte quantitation by using the standard addition technique. The procedure received ISO/IEC 17025:2005 accreditation from the American Association for Laboratory Accreditation under their Flexible Scope Policy. The developed validation protocol and associated procedural changes made to the daily operations are supported by several laboratory documents that are unique to the Flexible Scope option.


ISO/IEC 17025:2005 accreditation, pesticide misuse, FIFRA, state, Flexible Scope Policy

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