Elimination of Sodium Chloride Interference on Heavy Metal Analysis in High-Salt Food

Yabing Xiao, Jian He, Xing Ma, Haibin Zhang, Wei Li


A simple method using Chelex-100 chelating resin and Atomic Absorbance Spectroscopy determination of lead, cadmium and copper in high-salt food was established. This method could solve the problem of interference on the determination of lead and cadmium in salt matrix. Kd values of sodium (Na), lead (Pb), cadmium (Cd) and copper (Cu) on chelating resin was measured. The influence of pH values of solution on absorption and behavior of ions on resin were investigated. Elution curve for Na and Cd was demonstrated. Absorption capacities of resin to Pb/Cd/Cu/Na were determined. The developed method was applied for analysis of high-salt samples. The recoveries were in the range of 91.6%~98.6% with relative standard deviations (RSDs) in the range of 2.0%~6.7%.


Chelex-100, chelating resin, lead, cadmium, copper, sodium, high-salt food

Full Text:



Buruiana, D. L., Lefter, D., Tiron, G. L., Balta, S., & Bordei, M. (2015, June). Toxicity of Heavy Metals on the Environment and Human Health. Presented at the Proceedings of the 15th International Multidisciplinary Scientific Geo Conference SGEM, Albena, Bulgaria.

Zimmerman, A. J., & Weindorf, D. C. (2010). Heavy Metal and Trace Metal Analysis in Soil by Sequential Extraction: A Review of Procedures. International Journal of Analytical Chemistry, 2010, Article ID 387803.

Sumida, T., Nakazato, T., Tao, H., Oshima, M., & Motomizu, S. (2006). On-line Preconcentration System Using Mini-column Packed with a Chelating Resin for the Characterization of Seasonal Variations of Trace Elements in Seawater by ICP-MS and ICP-AES. Analytical Sciences, 22, 1163-1168.

Vegueria, S. F. J., Godoy, J. M., de Campos, R. C., & Goncalves, R. A. (2013). Trace element determination in seawater by ICP-MS using online, offline and bath procedures of preconcentration and matrix elimination. Microchemical Journal, 106, 121-128.

Kiptoo, J. K., Ngila, J. C., & Silavwe, N. D. (2009). Evaluation of copper speciation in model solutions of humic acid by mini-columns packed with Chelex-100 and new chelating agents: Application to speciation of selected heavy metals in environmental water samples. Journal of Hazardous Materials, 172, 1163-1167.

Wu, P., Yu, L., & Wang, H. (2011). Enrichment and Separation of Harmful Elements in Food and Its Application in Some Optical and Electrochemical Analysis Methods. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 47, 1243-1247.

Bowles, K. C., Apte, S. C., Batley, G. E., Hales, L. T., & Rogers, N. J. (2006). A rapid Chelex column method for the determination of metal speciation in natural waters. Analytica Chimica Acta, 558, 237-245.

Leinonen, H., & Lehto, J. (2000). Ion-exchange of nickel by iminodiacetic acid chelating resin Chelex 100. Reactive and Functional Polymers, 43, 1-6.

Manouchehri, N., & Bermond, A. (2006). Study of trace metal partitioning between soil-EDTA extracts and Chelex-100 resin. Analytica Chimica Acta, 557, 337-343.

Strelow, F. W. E. (1960). An Ion Exchange Selectivity Scale of Cations Based on Equilibrium Distribution Coefficients.Analytical Chemistry, 32, 1185-1188.