Analysis of Pesticides in Olive Oil Using a Modified QuEChERS Method with LC-MS/MS and GC-MS/MS

Narong Chamkasem, Tiffany Harmon


A simple and high-throughput screening method for the analysis of pesticides in olive oil is presented. A modified QuEChERS sample preparation method was developed to improve the extraction recovery of highly lipophilic pesticides. The acetonitrile extract of the olive oil was directly injected to LC-MS/MS, while other GC-amenable compounds were treated with the modified QuEChERS procedure for GC-MS/MS analysis. The method is an extension of the LIB 4517 to include olive oil. The average recoveries for 80 pesticides quantified by LC-MS/MS at 200, 500, and 1000 ng/g fortifying levels were 91% or better (RSD < 5.5%), while GC-MS/MS analysis demonstrated 81% or better (RSD < 7.2%) for average recovery from 59 compounds at the same spike levels. This method showed an improved recovery of several challenging lipophilic
pesticides in olive oils.


QuEChERS, Pesticides Analysis, LC-MS/MS, GC-MS/MS, Olive oil

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